The Extracellular Matrix Engineering Research Laboratory was started in April, 2005, by Dr. Jeffrey Ruberti. Though the lab comes under the Department of Mechanical Engineering, the research we conduct is more within the auspices of Biomedical Engineering.
Much of our research involves collagen, the fibrous protein constituent of bone, tissue, cartilage, tendon and other connective tissues. We are trying to determine:
The laboratory is located in the Egan Research Center on the main Boston campus of Northeastern University. There is approximately 700 sq. feet of laboratory space.
There are a two Nikon T2000-E microscopes, both of which are capable of Differential Interference Contrast microscopy. They both have electronic stages and Z movement capabilities and a range of objectives (10x - 60x). One is equipped with micro-pipettes, which allows the micromanipulation of individual collagen fibrils. We also have a light microscope, and access to a Hitachi Scanning Electron Microscope and a JEOL Transmission Electron Microscope.
We also have one of the original Cressington Quick Freeze Deep Etch (QFDE) machines in the laboratory. Using this machine in conjunction with TEM provides us with amazing imaging capabilities.
Included in this is a full cell culture room, where we culture our own bovine corneal fibroblasts (directly from bovine corneas) for experiments. We have a NuAir Laminar flow hood and Thermo water jacketed incubator and a small, portable autoclave.